In this video, DeNovix Application Scientist Ben Capozzoli explains how to count single nuclei suspensions on the CellDrop Automated Cell Counter.
Isolating nuclei is critical for a number of workflows, including ATAC-sequencing, RNA-sequencing, and a variety of others. To ensure successful results in downstream applications, it's important to have accurate counts of your nuclei and understand how much debris is in your sample.
The CellDrop efficiently determines nuclei counts as well as an extraction efficiency score, regardless of the presence of debris. Watch to learn more about how this process works.
Experience the CellDrop
Free Trial Program
https://www.denovix.com/free-trial/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Schedule a Virtual Demo
https://www.denovix.com/virtual-demo/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Resources
Tech Note 216: Counting Isolated Nuclei Using a CellDrop (detailed protocol, list of tissue types, etc.)
https://www.denovix.com/tn-216-counting-isolated-nuclei-using-celldrop/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Tech Note 186: CellDrop Best Practices (loading, adjusting focus & exposure, cleaning, etc.)
https://www.denovix.com/tn-186-celldrop-best-practices/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
CellDrop Protocol Settings (list of pre-configured protocols for different cell types)
https://www.denovix.com/products/celldrop-protocol-settings/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips#celldrop-protocols-nuclei
Contact us at info@denovix.com if you have any questions.
Chapters:
00:00 Introduction
00:46 The CellDrop Automated Cell Counter
01:15 Preparing the Nuclei Suspension
02:05 AO/PI Viability Dye
02:40 CellDrop Count & Results
03:46 Cleaning
03:58 Outro
Isolating nuclei is critical for a number of workflows, including ATAC-sequencing, RNA-sequencing, and a variety of others. To ensure successful results in downstream applications, it's important to have accurate counts of your nuclei and understand how much debris is in your sample.
The CellDrop efficiently determines nuclei counts as well as an extraction efficiency score, regardless of the presence of debris. Watch to learn more about how this process works.
Experience the CellDrop
Free Trial Program
https://www.denovix.com/free-trial/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Schedule a Virtual Demo
https://www.denovix.com/virtual-demo/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Resources
Tech Note 216: Counting Isolated Nuclei Using a CellDrop (detailed protocol, list of tissue types, etc.)
https://www.denovix.com/tn-216-counting-isolated-nuclei-using-celldrop/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
Tech Note 186: CellDrop Best Practices (loading, adjusting focus & exposure, cleaning, etc.)
https://www.denovix.com/tn-186-celldrop-best-practices/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips
CellDrop Protocol Settings (list of pre-configured protocols for different cell types)
https://www.denovix.com/products/celldrop-protocol-settings/?utm_source=youtube&utm_medium=video&utm_campaign=techteamtips#celldrop-protocols-nuclei
Contact us at info@denovix.com if you have any questions.
Chapters:
00:00 Introduction
00:46 The CellDrop Automated Cell Counter
01:15 Preparing the Nuclei Suspension
02:05 AO/PI Viability Dye
02:40 CellDrop Count & Results
03:46 Cleaning
03:58 Outro