Andy Jones on 01/03/2018

131 Diagnostics App Self Test

Diagnostics App Self Test Although the DS-11 propriety SmartPath® technology enables accurate pathlength control and eliminates the need for routine recalibration, labs may choose to run the Diagnostics app to verify that the instrument is working within specifications. The Self Test option within the app is specifically designed to assess parameters other than pathlength calibration.

A Andy Jones
Andy Jones on 01/03/2018

179 Fluorescein Performance Data

he DeNovix DS-11 FX+ and QFX series of fluorometers enable precise fluorescein quantification using a proprietary optical core and four versatile fluorescence channels. DeNovix’ preconfigured EasyApps® include fluorescence apps which enable the user to create additional assays for DNA, RNA, protein, and other sample types. These apps include an LED Wizard in the assay creation window which helps the user select the appropriate fluorescence channel.

A Andy Jones
Andy Jones on 01/03/2018

170 GFP Quantification Performance Data

Enhanced GFP (EGFP) is a GFP isoform that is optimized to exhibit improved spectral characteristics for use with mammalian cells. This isoform was used to collect data for this technical note, and has an excitation maximum at 488 nm and an emission maximum at 508 nm, as shown below in Figure 1. Green fluorescent protein (GFP) use is ubiquitous in cell and molecular biology as a reporter for gene expression and in many other applications. GFP is an intrinsically fluorescent protein that is easily detected using a fluorometer or fluorescence microscope.

A Andy Jones
Andy Jones on 01/03/2018

171 Qubit ssDNA Assay Performance Data

This note presents typical performance data measured on the DeNovix QFX Fluorometer using the Thermo Fisher Scientific Qubit® ssDNA assay. DeNovix DS-11 FX and QFX fluorometers enable accurate and specific quantification of nucleic acids, proteins and other biomolecules through fluorescence measurement. DeNovix fluorometers utilize a proprietary optical core and a versatile set of four fluorescence channels for excitation and emission detection of fluorophores. Instruments include preinstalled EasyApps® configured for easy quantification using common ssDNA, dsDNA, RNA, and protein fluorescence assays.

A Andy Jones
Andy Jones on 01/03/2018

169 Qubit RNA Assay Performance Data

This note presents typical performance data measured on the DeNovix QFX using the Thermo Fisher Scientific Qubit® RNA High Sensitivity (HS) and Broad Range (BR) assays. DeNovix DS-11 FX and QFX Fluorometers enable accurate and specific quantification of nucleic acids, proteins and other biomolecules through fluorescence measurement. This note presents typical performance data measured on the DeNovix QFX using the Thermo Fisher Scientific Qubit® RNA High Sensitivity (HS) and Broad Range (BR) assays.

A Andy Jones
Andy Jones on 01/03/2018

162 DeNovix Fluorescent dsDNA Assay Ranges

DeNovix fluorescence assays enable dsDNA quantitation over a broad range of concentrations using three distinct fluorescence assays kits.

A Andy Jones
Andy Jones on 01/03/2018

161 DeNovix Ultra High Sensitivity Assay Protocol for Qubit fluorometers

The DeNovix Ultra High Sensitivity Assay is available for use on Qubit® 2.0 and 3.0 fluorometers. The assay parameter files are available on the DeNovix website. The DeNovix dsDNA Ultra High Sensitivity Assay enables accurate detection of purified doublestranded DNA (dsDNA) samples with a detection range from 5 pg to 3 ng total mass per assay tube. This is equivalent to sample concentrations of 0.5 pg/ µL to 300 pg/µL.

A Andy Jones
Andy Jones on 01/03/2018

160 DeNovix High Sensitivity Assay Protocol for Qubit fluorometers

The DeNovix High Sensitivity Assay is available for use on Qubit® 2.0 and 3.0 fluorometers. The assay parameter files are available on the DeNovix website. The DeNovix dsDNA High Sensitivity Assay enables accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range from 100 pg to 250 ng total mass in 200 µL volumes. This equates to sample concentrations of 10 pg/µL to 250 ng/µL when using between 1-20 µL sample volumes in 200 µL assay volumes. The assay is linear for sample concentrations as high as 250 ng/µL when adjusting volumes to 1 µL of sample into 199 µL of working reagent. Total mass should not exceed 250 ng for best results.

A Andy Jones
Andy Jones on 01/03/2018

147 Ultra High Sensitivity Assay Protocol

dsDNA Ultra High Sensitivity Assay Protocol The DeNovix dsDNA Ultra High Sensitivity Assay enables accurate detection of purified doublestranded DNA (dsDNA) samples with a detection range from 5 pg to 3 ng total mass per assay tube. This is equivalent to sample concentrations of 0.5 pg/ µL to 300 pg/µL.

A Andy Jones
Andy Jones on 01/03/2018

146 Ultra High Sensitivity Assay Short Protocol

dsDNA Ultra High Sensitivity Assay Short Protocol The DeNovix dsDNA Ultra High Sensitivity Assay enables accurate detection of purified doublestranded DNA (dsDNA) samples with a detection range from 5 pg to 3 ng total mass per assay tube. This is equivalent to sample concentrations of 0.5 pg/ µL to 300 pg/µL.

A Andy Jones
Andy Jones on 01/03/2018

145 High Sensitivity Assay Protocol

dsDNA High Sensitivity Assay Protocol The DeNovix dsDNA High Sensitivity Assay enables accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range from 100 pg to 250 ng total mass in 200 µL volumes. This equates to sample concentrations of 10 pg/µL to 250 ng/µL when using between 1-20 µL sample volumes in 200 µL assay volumes. The assay is linear for sample concentrations as high as 250 ng/µL when adjusting volumes to 1 µL of sample into 199 µL of working reagent. Total mass should not exceed 250 ng for best results.

A Andy Jones
Andy Jones on 01/03/2018

144 High Sensitivity Assay Short Protocol

dsDNA High Sensitivity Assay Short Protocol The DeNovix dsDNA High Sensitivity Assay enables accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range from 100 pg to 250 ng total mass in 200 µL volumes. This equates to sample concentrations of 10 pg/µL to 250 ng/µL when using between 1-20 µL sample volumes in 200 µL assay volumes. The assay is linear for sample concentrations as high as 250 ng/µL when adjusting volumes to 1 µL of sample into 199 µL of working reagent. Total mass should not exceed 250 ng for best results.

A Andy Jones
Andy Jones on 01/03/2018

143 Broad Range Assay Protocol

The DeNovix dsDNA Broad Range Assay enables accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range from 2 to 2000 ng total mass in 200 µL volumes. This equates to sample concentrations of 0.1 ng/µL to 2000 ng/µL when using 1 to 20 µl

A Andy Jones
Andy Jones on 01/03/2018

142 Broad Range Assay Short Protocol

dsDNA Broad Range Assay Short Protocol The DeNovix dsDNA Broad Range Assay enables accurate detection of double-stranded DNA (dsDNA) samples with a standard detection range from 2 to 2000 ng total mass in 200 µL volumes. This equates to sample concentrations of 0.1 ng/µL to 2000 ng/µL when using 1 to 20 µL sample volumes in a 200 µL total assay volume.

A Andy Jones
Andy Jones on 01/03/2018

156 Absorbance and Fluorescence Quantification

The purpose of this technical document is to describe and compare the complementary methods of absorbance and fluorescence quantification. DeNovix DS-11 FX Spectrophotometer / Fluorometer Series instruments enable precise absorbance and fluorescence quantification across a wide dynamic range. The dual mode spectrophotometer incorporates SmartPath® Technology, which facilitates accurate and reproducible measurements for both cuvette and 1 µL absorbance modes. The proprietary optical core of the fluorescence component utilizes LED excitation sources and highly sensitive photodiodes capable of detecting minute amounts of fluorescence across four versatile wavelength ranges.

A Andy Jones
Andy Jones on 01/03/2018

154 Fluorescence Measurements Best Practices

This tech note provides some best practice tips for making fluorescent measurements using the DeNovix DS-11 FX or QFX series of fluorometers. Fluorometric quantitation assays for nucleic acids and protein are now routine in many life science research labs. As with many fluorescence techniques, it is important to apply best practices to ensure reliable results and reduce user error. This tech note provides some best practice tips for making fluorescent measurements using the DeNovix DS-11 FX or QFX series of fluorometers.

A Andy Jones
Andy Jones on 01/03/2018

176 Methods of Protein Quantification

The purpose of this technical document is to describe and compare the methods to quantify proteins using a DeNovix Spectrophotometer / Fluorometer Series instrument. DeNovix DS-11 FX Spectrophotometer / Fluorometer Series instruments enable precise absorbance and fluorescence quantification of proteins across a wide dynamic range. The DS-11 FX+ includes 3 measurement modes in one instrument: microvolume absorbance, cuvette absorbance, and fluorescence. Each of the 3 modes enables quantification of different types of protein samples including but not limited to purified protein, cell or tissue extracts, and low concentration samples.

A Andy Jones
Andy Jones on 01/03/2018

172 DS-11 FX Series vs Qubit®

This note presents a comparison of instrument features and system performance of the DS-11 FX Series instruments and the Qubit 3.0. A performance comparison of DeNovix dsDNA quantification assays and Qubit dsDNA assays is also presented. The DeNovix DS-11 FX Series instruments and the Qubit 3.0 Fluorometer are commonly used for fluorescence quantification of nucleic acids, proteins and other biomolecules. This note presents a comparison of instrument features and system performance of the DS-11 FX Series instruments and the Qubit 3.0. A performance comparison of DeNovix dsDNA quantification assays and Qubit dsDNA assays is also presented.

A Andy Jones
Andrew Jones on 12/22/2017

116 Malware Protection

A natural question regarding DeNovix® software is whether it can be infected by a software virus. The simple answer is no. Android apps work under a “sandboxed” security scheme which is a technique that places “virtual walls” between apps and the rest of the device’s software. A natural question regarding DeNovix® software is whether it can be infected by a software virus. The simple answer is no. Android apps work under a “sandboxed” security scheme which is a technique that places “virtual walls” between apps and the rest of the device’s software.

  Andrew Jones
Andrew Jones on 12/22/2017

118 Software Updates via USB

The Updater app is used to automatically install all apps that require updating. The new software may be installed from a flash drive or directly from the web. The Updater app is used to automatically install all apps that require updating. The new software may be installed from a flash drive or directly from the web.

  Andrew Jones