Webinar | Optimizing Nuclei Extraction & Counting for Single Cell Sequencing

Posted on 09/25/2023
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DeNovix Application Scientist Ben Capozzoli discusses methods and tips for preparing nuclei samples before single cell sequencing workflows.

Our featured method for achieving accurate, reproducible nuclei counts is pairing the CellDrop™ Automated Cell Counter with AO/PI fluorescent label. Ben performs a short demonstration of an isolated nuclei count using the CellDrop's dedicated Nuclei AO/PI app.

For a summary of the important points discussed during this webinar, download our free infographic: https://www.denovix.com/counting-and-quality-control-of-isolated-nuclei-infographic/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_sep_2023

Learn more about how the CellDrop can improve your nuclei counting workflow:
Virtual Demo: https://www.denovix.com/virtual-demo/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_sep_2023
Free Trial: https://www.denovix.com/free-trial/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_sep_2023
Product Information: https://www.denovix.com/celldrop-quick-facts/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_sep_2023

00:00 Introduction
02:57 Poll 1
04:21 Webinar Map
05:46 Tissue Prep
06:40 Nuclei Extraction
09:50 Sample Prep
12:55 Intro to CellDrop™
15:51 Counting Protocol
18:46 Imaging and Nuclei Counting
23:21 Poll 2
24:36 Live Demo
33:58 Additional Tissues
38:16 Downstream Applications
39:13 Summary
42:15 Q&A Session
56:50 Closing