Trypan Blue is commonly used in cell culture labs for selectively staining dead cells and tissues. However, fluorescence cell counting methods offer improved accuracy for many applications, including challenging sample types like primary cells and isolated nuclei.
In this webinar, DeNovix Scientific Director Dan Schieffer explains the advantages of fluorescence for cell counting and viability assessment. Dan compares how brightfield and fluorescence viability dyes work, demonstrates several types of measurements using the CellDrop Automated Cell Counter, and answers audience questions during a live Q&A session.
This webinar was co-hosted by Elvis Pérez of Bonsai Lab, the distributor of DeNovix instruments in Spain and Portugal.
Experience the CellDrop
Free Trial Program
https://www.denovix.com/free-trial/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_jun_2023
Schedule a Virtual Demo
https://www.denovix.com/virtual-demo/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_jun_2023
Chapters
00:00 Introduction
01:01 Agenda
01:53 Poll
02:12 DeNovix Overview
03:01 Poll Results
03:33 CellDrop Automated Cell Counter
04:54 Live Demo: Tissue Culture with Trypan Blue
07:44 DirectPipette™ Technology
09:27 Choosing the Right Method
10:34 How Trypan Blue Works
11:32 How AO/PI Works
13:28 Why Use Fluorescence?
14:38 Live Demo: Whole Blood & Nuclei with AO/PI
20:05 Case Study: Trypan Blue vs. AO/PI
21:13 Other Fluorescence Applications
22:16 Live Demo: Data Analysis & Exporting
24:01 Summary
25:28 Q&A Session
29:57 Outro
In this webinar, DeNovix Scientific Director Dan Schieffer explains the advantages of fluorescence for cell counting and viability assessment. Dan compares how brightfield and fluorescence viability dyes work, demonstrates several types of measurements using the CellDrop Automated Cell Counter, and answers audience questions during a live Q&A session.
This webinar was co-hosted by Elvis Pérez of Bonsai Lab, the distributor of DeNovix instruments in Spain and Portugal.
Experience the CellDrop
Free Trial Program
https://www.denovix.com/free-trial/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_jun_2023
Schedule a Virtual Demo
https://www.denovix.com/virtual-demo/?utm_source=youtube&utm_medium=video&utm_campaign=webinar_jun_2023
Chapters
00:00 Introduction
01:01 Agenda
01:53 Poll
02:12 DeNovix Overview
03:01 Poll Results
03:33 CellDrop Automated Cell Counter
04:54 Live Demo: Tissue Culture with Trypan Blue
07:44 DirectPipette™ Technology
09:27 Choosing the Right Method
10:34 How Trypan Blue Works
11:32 How AO/PI Works
13:28 Why Use Fluorescence?
14:38 Live Demo: Whole Blood & Nuclei with AO/PI
20:05 Case Study: Trypan Blue vs. AO/PI
21:13 Other Fluorescence Applications
22:16 Live Demo: Data Analysis & Exporting
24:01 Summary
25:28 Q&A Session
29:57 Outro